High Performance 3D-bioprinted Biofunctionalized ADA-GEL Containing Cu-MBGs for Bone Tissue Engineering
Nowadays, the significant number of bone fractures due to osteoporosis represents a relevant worldwide problem still unsolved. Bone substitutes that provides a permanent or ideally temporary porous device (scaffold) with angiogenesis, osteogenesis and chronic wound healing ability can allow repair mechanisms to take place. 3D-bioprinting is an attractive technique that utilizes a layer-by-layer method for accurate fabrication of biomaterial scaffolds with tunable properties.
Therefore, the aim of this study is to develop novel biofunctionalized materials used for hard tissue repair, with high printing performance, bioactivity, angiogenesis capability and biocompatibility. ADA-GEL with superior cell adhesion properties and controllable degradation characteristic was prepared by covalent crosslinking of ADA and gelatin . Cationic-modified copper-doped mesoporous bioactive glass nanoparticles (MBGNs)  were than incorporated into those partly crosslinked ADA-GEL to further improve the printing fidelity of inks and endow it functional properties as well . Various parameters such as chemical composition and surface chemistry of Cu-/ MBGNs were than optimized and characterized in terms of physic-chemical and rheological properties. Afterwards, porous scaffolds were fabricated by extrusion of those inks and post-crosslinking in CaCl2 solution. In vitro bioactivity, cell compatibility and VEGF releasing tests were performed on those scaffolds and results were analyzed by using ADA-GEL scaffolds as positive control.
It was shown that those novel inks exhibit strong printability and fidelity, through the combination of cationic particles and anionic hydrogel. In vitro mineralization could be observed by SEM, showing the promising bioactivity of the scaffolds. Additionally, the preliminary cell test confirmed the biocompatibility of these materials.
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